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KMID : 0385220000100010020
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Korean Journal of Gerontology
2000 Volume.10 No. 1 p.20 ~ p.25
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Vitamin E dose-Associated Alterations in Mouse Splenocyte Proliferation
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Lee Ji-Hea
Lee You-Sook
Kim Hyun-Sook
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Abstract
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Evidences from animal and human trials suggested that the association between vitamin E and immune function. In this study, we investigated the vitamin E dose-associated alteration in mouse immunity. Two different doses of vitamin E, 20 IU vitamin E/kg b.w. for optimal supplement group and 80 IU vitamin E/kg b.w. far overdose group, were injected daily into the intraperitoneal cavity for 14 days in male ICR mouse. Arouse splenocyte proliferation against the mitogen stimulation such as Con A, LPS, or PWM of each group was assessed as an index of immune function, and compared with that of control group injected with the same volume of saline. Serum vitamin E concentration was analyzed by HPLC to assure that intraperitoneally injected vitamin E was appropriately absorbed. The animals in the high dose of vitamin E supplement group showed significantly lower body weight gain and spleen index compared to those in optimum level of vitamin E supplement group. More importantly, while the splenocyte proliferation of optimal supplement vitamin E group resulted to be enhanced significantly, the overdose vitamin E injected animals showed suppressed proliferation capacity compared to that in control animals. Consequently, optimum supplementation of vitamin E would be required for the proper capacity of immunity, whereas overdose of vitamin E supplementation may rather impair the immunocompetence.
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KEYWORD
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Vitamin E, Splenocyte Proliferation, Immunocompetence
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